Comparing the susceptibility of genetically modified mice to intoxication by organophosphorus nerve agents

Genetically modified mice- Methods, applications and outlook

Background & Aim: Transgenic mice, of tengenerated by random integration of foreign genes into the mouse genome or by targeted mutation in a particular gene, have demonstrated to be a very effective tool for studying gene function in living things. In this review article, we discussed on the current methods of generating genetically-modified mice and their related problems and then investigated...

Modified Cholinesterase Technology in the Construction of Biosensors for Organophosphorus Nerve Agents and Pesticides Detection

Organophosphorus compounds are a wide-spread group of agents which can be used among others as pesticides, especially insecticides, or chemical warfare agents. These extremely toxic compounds irreversibly inhibit the enzymes of hydrolases class, which have the catalytic capability of hydrolyzing their specific neurotransmitters in synaptic clefts of the nervous system. The organophosphorus pest...

Effective countermeasure against poisoning by organophosphorus insecticides and nerve agents.

The nerve agents soman, sarin, VX, and tabun are deadly organophosphorus (OP) compounds chemically related to OP insecticides. Most of their acute toxicity results from the irreversible inhibition of acetylcholinesterase (AChE), the enzyme that inactivates the neurotransmitter acetylcholine. The limitations of available therapies against OP poisoning are well recognized, and more effective anti...

Immobilization of organophosphorus hydrolase for the degradation of organophosphorus nerve agents.

A Disposable Biosensor for Organophosphorus Nerve Agents Based on Carbon Nanotubes Modified Thick Film Strip Electrode

A disposable biosensor based on acetylcholinesterase-functionalized acid purified multi-wall carbon nanotubes (CNTs) modified thick film strip electrode for organophosphorus (OP) insecticides was developed. The degree of inhibition of the enzyme acteylcholinesterase (AChE) by OP compounds was determined by measuring the electrooxidation current of the thiocholine generated by the AChE catalyzed...